Bis-spirolabdane diterpenoids from Leonurus japonicus and their anti-platelet aggregative activity

Abstract

Six bis-spirolabdane diterpenoids along with four known analogues were isolated from the aerial parts of Leonurus japonicus. Their structures and absolute configurations were elucidated by spectroscopic analyses, single-crystal X-ray diffraction, and a modified Mosher's method. The inhibitory activity of the compounds against the abnormal increase in platelet aggregation induced by adenosine diphosphate was investigated. Only the (13R)-bis-spirolabdane diterpenoids exhibited a significant effect.

Keywords: Absolute configuration; Anti-platelet aggregative activity; Bis-spirolabdane diterpenoids; Leonurus japonicus.