Abstract
miR-320 expression level is found to be down-regulated in human colon cancer. To date, however, its underlying mechanisms in the chemo-resistance remain largely unknown. In this study, we demonstrated that ectopic expression of miR-320 led to inhibit HCT-116 cell proliferation, invasion and hypersensitivity to 5-Fu and Oxaliplatin. Also, knockdown of miR-320 reversed these effects in HT-29 cells. Furthermore, we identified an oncogene, FOXM1, as a direct target of miR-320. In addition, miR-320 could inactive the activity of Wnt/β-catenin pathway. Finally, we found that miR-320 and FOXM1 protein had a negative correlation in colon cancer tissues and adjacent normal tissues. These findings implied that miR-320-FOXM1 axis may overcome chemo-resistance of colon cancer cells and provide a new therapeutic target for the treatment of colon cancer.
Keywords:
Chemo-resistance; Colon cancer; FOXM1; Wnt; miR-320.
Copyright © 2014 Elsevier Inc. All rights reserved.
MeSH terms
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3' Untranslated Regions / genetics
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Apoptosis / drug effects
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Apoptosis / genetics
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Base Sequence
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Cell Cycle / drug effects
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Cell Cycle / genetics
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Cell Movement / drug effects
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Cell Movement / genetics
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Cell Proliferation / drug effects
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Cell Proliferation / genetics
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Chemoradiotherapy*
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Colonic Neoplasms / genetics*
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Colonic Neoplasms / pathology
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Colonic Neoplasms / therapy*
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Fluorouracil / pharmacology
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Fluorouracil / therapeutic use
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Forkhead Box Protein M1
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Forkhead Transcription Factors / genetics
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Forkhead Transcription Factors / metabolism*
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Gene Expression Regulation, Neoplastic / drug effects
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HCT116 Cells
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HT29 Cells
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Humans
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MicroRNAs / genetics
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MicroRNAs / metabolism*
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Molecular Sequence Data
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Neoplasm Invasiveness
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Organoplatinum Compounds / pharmacology
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Organoplatinum Compounds / therapeutic use
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Oxaliplatin
Substances
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3' Untranslated Regions
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FOXM1 protein, human
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Forkhead Box Protein M1
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Forkhead Transcription Factors
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MIRN320 microRNA, human
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MicroRNAs
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Organoplatinum Compounds
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Oxaliplatin
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Fluorouracil