Application of PCDA/SPH/CHO/Lysine vesicles to detect pathogenic bacteria in chicken

Taíla V de Oliveira; Nilda de F F Soares; Nélio J de Andrade; Deusanilde J Silva; Eber Antônio A Medeiros; Amanda T Badaró

doi:10.1016/j.foodchem.2014.09.055

Application of PCDA/SPH/CHO/Lysine vesicles to detect pathogenic bacteria in chicken

Food Chem. 2015 Apr 1:172:428-32. doi: 10.1016/j.foodchem.2014.09.055. Epub 2014 Sep 19.

Authors

Taíla V de Oliveira¹, Nilda de F F Soares², Nélio J de Andrade³, Deusanilde J Silva⁴, Eber Antônio A Medeiros⁵, Amanda T Badaró⁶

Affiliations

¹ Food Technology Department, Universidade Federal de Viçosa, Avenida PH Holfs, s/n, Campus Universitário, 36.570-000 Viçosa, Minas Gerais, Brazil. Electronic address: taveloso@yahoo.com.br.
² Food Technology Department, Universidade Federal de Viçosa, Avenida PH Holfs, s/n, Campus Universitário, 36.570-000 Viçosa, Minas Gerais, Brazil. Electronic address: nfsoares@ufv.br.
³ Food Technology Department, Universidade Federal de Viçosa, Avenida PH Holfs, s/n, Campus Universitário, 36.570-000 Viçosa, Minas Gerais, Brazil. Electronic address: nandrade@ufv.br.
⁴ Chemistry Engineering Department, Universidade Federal de Viçosa, Avenida PH Holfs, s/n, Campus Universitário, 36.570-000 Viçosa, Minas Gerais, Brazil. Electronic address: deusanilde@gmail.com.
⁵ Food Technology Department, Universidade Federal de Viçosa, Avenida PH Holfs, s/n, Campus Universitário, 36.570-000 Viçosa, Minas Gerais, Brazil. Electronic address: ebermedeiros@yahoo.com.br.
⁶ Food Technology Department, Universidade Federal de Viçosa, Avenida PH Holfs, s/n, Campus Universitário, 36.570-000 Viçosa, Minas Gerais, Brazil. Electronic address: amanda.badaro@ufv.br.

PMID: 25442574
DOI: 10.1016/j.foodchem.2014.09.055

Abstract

During the course of infection, Salmonella must successively survive the harsh acid stress of the stomach and multiply into a mild acidic compartment within macrophages. Inducible amino acid decarboxylases are known to promote adaptation to acidic environments, as lysine decarboxylation to cadaverine. The idea of Salmonella defenses responses could be employed in systems as polydiacetylene (PDA) to detect this pathogen so important to public health system. Beside that PDA is an important substance because of the unique optical property; that undergoes a colorimetric transitions by various external stimuli. Therefore 10,12-pentacosadyinoic acid (PCDA)/Sphingomyelin(SPH)/Cholesterol(CHO)/Lysine system was tested to determine the colorimetric response induced by Salmonella choleraesuis. PCDA/SPH/CHO/Lysine vesicles showed a colour change even in low S. choleraesuis concentration present in laboratory conditions and in chicken meat. Thus, this work showed a PCDA/SPH/CHO/Lysine vesicle application to simplify routine analyses in food industry, as chicken meat industry.

Keywords: Chicken meat; Limit detection of Salmonella choleraesuis; Polydiacetylene vesicle.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Chickens
Cholesterol / chemistry
Colorimetry*
Food Microbiology / methods*
Hydrogen-Ion Concentration
Liposomes / chemistry*
Lysine / chemistry
Meat / microbiology
Polyacetylene Polymer
Polymers / chemistry
Polyynes / chemistry
Salmonella enterica / isolation & purification*
Sphingomyelins / chemistry

Substances

Liposomes
Polyacetylene Polymer
Polymers
Sphingomyelins
Polyynes
polydiacetylene
Cholesterol
Lysine