Gene dynamics of core transcription factors for pluripotency in embryonic stem cells

Brit G David; Kazuko Okamoto; Taishi Kakizuka; Taro Ichimura; Tomonobu M Watanabe; Hideaki Fujita

doi:10.1016/j.jbiosc.2014.09.011

Gene dynamics of core transcription factors for pluripotency in embryonic stem cells

J Biosci Bioeng. 2015 Apr;119(4):406-9. doi: 10.1016/j.jbiosc.2014.09.011. Epub 2014 Nov 3.

Authors

Brit G David¹, Kazuko Okamoto², Taishi Kakizuka¹, Taro Ichimura², Tomonobu M Watanabe³, Hideaki Fujita⁴

Affiliations

¹ Graduate School of Frontier Bioscience, Osaka University, 1-3 Yamadaoka, Suita, Osaka 565-0871, Japan.
² Quantitative Biology Center (QBiC), Riken, Suita, Osaka 565-0874, Japan.
³ Graduate School of Frontier Bioscience, Osaka University, 1-3 Yamadaoka, Suita, Osaka 565-0871, Japan; Quantitative Biology Center (QBiC), Riken, Suita, Osaka 565-0874, Japan.
⁴ Graduate School of Frontier Bioscience, Osaka University, 1-3 Yamadaoka, Suita, Osaka 565-0871, Japan; Quantitative Biology Center (QBiC), Riken, Suita, Osaka 565-0874, Japan; WPI, Immunology Frontier Research Center (IFReC), Osaka University, Suita, Osaka 565-0871, Japan. Electronic address: hideaki.fujita@riken.jp.

PMID: 25441442
DOI: 10.1016/j.jbiosc.2014.09.011

Abstract

Embryonic stem cell (ESC) pluripotency is maintained by core transcription factors (TFs). Although the expression of these TFs is well documented, their expression dynamics is poorly evaluated. Here, we visualized the dynamics of Nanog and Oct3/4 expression in ESC using fluorescent reporters and found that expression of these TFs change dramatically during culture.

Keywords: Embryonic stem cell; Imaging; Nanog; Oct3/4; Sox2.

MeSH terms

Animals
Cell Culture Techniques
Cells, Cultured
Embryonic Stem Cells / cytology
Embryonic Stem Cells / metabolism*
Genes, Reporter / genetics
Homeodomain Proteins / genetics
Homeodomain Proteins / metabolism
Mice
Nanog Homeobox Protein
Octamer Transcription Factor-3 / genetics
Octamer Transcription Factor-3 / metabolism
Pluripotent Stem Cells / cytology
Pluripotent Stem Cells / metabolism*
SOXB1 Transcription Factors / genetics
SOXB1 Transcription Factors / metabolism
Transcription Factors / genetics*
Transcription Factors / metabolism

Substances

Homeodomain Proteins
Nanog Homeobox Protein
Nanog protein, mouse
Octamer Transcription Factor-3
SOXB1 Transcription Factors
Sox2 protein, mouse
Transcription Factors