Tandem measurements of iron and creatinine by cross injection analysis with application to urine from thalassemic patients

N Choengchan; T Mantim; P Inpota; D Nacapricha; P Wilairat; P Jittangprasert; W Waiyawat; S Fucharoen; P Sirankpracha; N Phumala Morales

doi:10.1016/j.talanta.2014.04.090

Tandem measurements of iron and creatinine by cross injection analysis with application to urine from thalassemic patients

Talanta. 2015 Feb:133:52-8. doi: 10.1016/j.talanta.2014.04.090. Epub 2014 Jul 7.

Authors

N Choengchan¹, T Mantim², P Inpota³, D Nacapricha², P Wilairat², P Jittangprasert⁴, W Waiyawat², S Fucharoen⁵, P Sirankpracha⁵, N Phumala Morales⁶

Affiliations

¹ Flow Innovation-Research for Science and Technology Laboratories (FIRST Labs), Bangkok, Thailand; Department of Chemistry and the Applied Analytical Chemistry Research Unit, Faculty of Science, King Mongkut׳s Institute of Technology, Ladkrabang, Chalongkrung Road, Bangkok 10520, Thailand. Electronic address: nchoengchan@gmail.com.
² Flow Innovation-Research for Science and Technology Laboratories (FIRST Labs), Bangkok, Thailand; Center of Excellence for Innovation in Chemistry and Department of Chemistry, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok 10400, Thailand.
³ Flow Innovation-Research for Science and Technology Laboratories (FIRST Labs), Bangkok, Thailand; Department of Chemistry and the Applied Analytical Chemistry Research Unit, Faculty of Science, King Mongkut׳s Institute of Technology, Ladkrabang, Chalongkrung Road, Bangkok 10520, Thailand.
⁴ Flow Innovation-Research for Science and Technology Laboratories (FIRST Labs), Bangkok, Thailand; Department of Chemistry, Faculty of Science, Srinakharinwirot University, Sukhumvit 23 Road, Bangkok 10110, Thailand.
⁵ Thalassemia Research Center, Institute of Molecular Biosciences, Mahidol University, Nakhonpathom 73170, Thailand.
⁶ Department of Pharmacology, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok 10400, Thailand.

PMID: 25435226
DOI: 10.1016/j.talanta.2014.04.090

Abstract

This work presents development of a method for the dual determination of Fe(III) and creatinine using cross injection analysis (CIA). Two CIA platforms connected in series accommodated sample and reagents plugs aspirated via y-direction channels while water was pumped through the x-direction channel toward a flow-through cell of a diode array UV-vis. detector. Iron was detected from the colorimetric reaction between Fe(II) and 2-(5-bromo-2-pyridylazo)-5-(N-propyl-N-(3-sulfopropyl)amino) aniline (5-Br-PSAA), with prior reduction of Fe(III) to Fe(II) by ascorbic acid. The Jaffe's reaction was employed for the detection of creatinine. Under the optimal conditions, good linearity ranges were achieved for iron in the range 0.5 to 7 mg L(-1) and creatinine in the range 50 to 800 mg L(-1). The CIA system was applied to spot urine samples from thalassemic patients undergoing iron chelation therapy, and was successfully validated with ICP-OES and batchwise Jaffe's method. Normalization of urinary iron excretion with creatinine is useful for correcting the iron concentration between urine samples due to variation of the collected urine volume.

Keywords: Creatinine; Cross injection analysis; Iron; Tandem measurements; Urine.

Publication types

Research Support, Non-U.S. Gov't
Validation Study

MeSH terms

Azo Compounds / chemistry
Colorimetry / instrumentation
Creatinine / urine*
Deferiprone
Equipment Design
Ferric Compounds / urine*
Flow Injection Analysis / instrumentation
Humans
Iron / urine*
Iron Chelating Agents / chemistry
Limit of Detection
Pyridones / chemistry
Thalassemia / urine*
Urinalysis / instrumentation*

Substances

Azo Compounds
Ferric Compounds
Iron Chelating Agents
Pyridones
Deferiprone
2-(5-bromo-2-pyridylazo)-5-(N-propyl-N-sulfopropylamino)aniline
Creatinine
Iron