Langmuir monolayers and thin films of amphifilic thiacalix[4]arenes. Properties and matrix for the immobilization of cytochrome c

Svetlana E Solovieva; Roman A Safiullin; Evgeni N Kochetkov; Nina B Melnikova; Marsil K Kadirov; Elena V Popova; Igor S Antipin; Alexander I Konovalov

doi:10.1021/la504379v

Langmuir monolayers and thin films of amphifilic thiacalix[4]arenes. Properties and matrix for the immobilization of cytochrome c

Langmuir. 2014 Dec 23;30(50):15153-61. doi: 10.1021/la504379v. Epub 2014 Dec 12.

Authors

Svetlana E Solovieva¹, Roman A Safiullin, Evgeni N Kochetkov, Nina B Melnikova, Marsil K Kadirov, Elena V Popova, Igor S Antipin, Alexander I Konovalov

Affiliation

¹ A.E. Arbuzov Institute of Organic and Physical Chemistry, Russian Academy of Sciences , Arbuzov st. 8, Kazan 420088, Russia.

PMID: 25435075
DOI: 10.1021/la504379v

Abstract

Formation and properties of Langmuir films of thiacalix[4]arene (TCA) derivatives containing N-donor groups on the lower rim (Y═O(CH2)3CN; OCH2CN; NH2; OCH2ArCN-p) in 1,3-alternate conformation on aqueous subphase and solid substrates have been studied. Only tetra-cyanopropoxy-p-tert-butylthiacalix[4]arene 1 forms a typical monomolecular layer with perpendicular orientation of the macrocycle relative to the water-air interface that is able to immobilize cytochrome c in the entire range of the surface pressure. Obtained monolayers were transferred by Langmuir-Schaefer technique onto quartz, indium-tin oxide (ITO), and silicon. It was demonstrated that protein activity is retained after immobilization on the substrate.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cattle
Cytochromes c / chemistry*
Enzymes, Immobilized / chemistry*
Hydrophobic and Hydrophilic Interactions*
Models, Molecular
Molecular Conformation
Phenols / chemistry*
Sulfides / chemistry*
Surface Properties
Water / chemistry

Substances

Enzymes, Immobilized
Phenols
Sulfides
thiacalix(4)arene
Water
Cytochromes c