Hes1 is upregulated by ischemic postconditioning and contributes to cardioprotection

Cell Biochem Funct. 2014 Dec;32(8):730-6. doi: 10.1002/cbf.3078. Epub 2014 Nov 28.

Abstract

The expression of Hes1 is increased following myocardial infarct and other ischemic cardiomyopathies, but the role of Hes1 in cardioprotection provided by ischemic postconditioning (IPost) remains unclear. In this study, we used gain and loss of function approaches to investigate the role of Hes1 in cardioprotection during IPost. Primary cardiac myocytes exposed to ischemia reperfusion injury (IRI) and IPost were used as the experimental model. The results showed that Hes1 expression was increased during myocardial IPost, and Hes1 promoted the viability while inhibited the apoptosis of cardiomyocytes. Moreover, Hes1 inhibited the opening of mitochondrial permeability transition pore (mPTP) and the generation of reactive oxygen species in primary cardiac myocytes exposed to IRI. Mechanistically, we found that Hes1-mediated cardioprotection was related to the downregulation of phosphatase and tensin homolog and the activation of phosphatidylinositol 3-kinase/Akt and signal transducer and activator of transcription 3 signalling. These data demonstrate that Hes1 is upregulated and mediates cardioprotection provided by IPost and suggest that Hes1 is a potential new target for the treatment of ischemic cardiomyopathy.

Keywords: Akt; Hes1; Stat3; ischemic postconditioning; mitochondrial permeability transition pore.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Newborn
  • Apoptosis
  • Basic Helix-Loop-Helix Transcription Factors / genetics
  • Basic Helix-Loop-Helix Transcription Factors / metabolism*
  • Cell Survival
  • Homeodomain Proteins / genetics
  • Homeodomain Proteins / metabolism*
  • Ischemic Postconditioning*
  • Mitochondrial Membrane Transport Proteins / metabolism
  • Mitochondrial Permeability Transition Pore
  • Myocardium / metabolism
  • Myocardium / pathology*
  • Myocytes, Cardiac / metabolism
  • PTEN Phosphohydrolase / metabolism
  • Primary Cell Culture
  • Rats, Sprague-Dawley
  • Reactive Oxygen Species / metabolism
  • Transcription Factor HES-1
  • Up-Regulation

Substances

  • Basic Helix-Loop-Helix Transcription Factors
  • Hes1 protein, rat
  • Homeodomain Proteins
  • Mitochondrial Membrane Transport Proteins
  • Mitochondrial Permeability Transition Pore
  • Reactive Oxygen Species
  • Transcription Factor HES-1
  • PTEN Phosphohydrolase
  • Pten protein, rat