Abstract
Here, we present a detailed protocol for the detection of activated caspase-8 in axotomized axons of the whole-mounted lamprey spinal cord. This method is based on the use of fluorochrome -labeled inhibitors of caspases (FLICA) in ex vivo tissue. We offer a very convenient vertebrate model to study the retrograde degeneration of descending pathways after spinal cord injury.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Axons / enzymology*
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Axons / pathology
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Caspase 8 / genetics*
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Caspase 8 / isolation & purification
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Caspase Inhibitors*
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Disease Models, Animal
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Fluorescent Dyes
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Lampreys
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Molecular Biology / methods
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Spinal Cord Injuries / diagnosis
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Spinal Cord Injuries / enzymology*
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Spinal Cord Injuries / pathology
Substances
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Caspase Inhibitors
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Fluorescent Dyes
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Caspase 8