Comparative study of conductometric glucose biosensor based on gold and on magnetic nanoparticles

Mater Sci Eng C Mater Biol Appl. 2013 Jan 1;33(1):298-303. doi: 10.1016/j.msec.2012.08.043. Epub 2012 Sep 11.

Abstract

The aim of this study was to show the feasibility and the performances of nanoparticle biosensing. A glucose conductometric biosensor was developed using two types of nanoparticles (gold and magnetic), glucose oxidase (GOD) being adsorbed on PAH (poly(allylamine hydrochloride)) modified nanoparticles, deposited on a planar interdigitated electrode (IDEs). The best sensitivities for glucose detection were obtained with magnetic nanoparticles (70 μM/mM and 3 μM of detection limit) compared to 45 μM/mM and 9 μM with gold nanoparticles and 30 μM/mM and 50 μM with GOD directly cross-linked on IDEs. When stored in phosphate buffer (20 mM, pH 7.3) at 4 °C, the biosensor showed good stability for more than 12 days.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biosensing Techniques
  • Conductometry*
  • Electrodes
  • Enzymes, Immobilized / chemistry
  • Enzymes, Immobilized / metabolism
  • Glucose / analysis*
  • Glucose Oxidase / chemistry
  • Glucose Oxidase / metabolism
  • Gold / chemistry*
  • Magnetite Nanoparticles / chemistry*
  • Magnetite Nanoparticles / ultrastructure
  • Microscopy, Electron, Transmission
  • Polyamines / chemistry

Substances

  • Enzymes, Immobilized
  • Magnetite Nanoparticles
  • Polyamines
  • polyallylamine
  • Gold
  • Glucose Oxidase
  • Glucose