A microsystem integrating photodegradable hydrogel microstructures and reconfigurable microfluidics for single-cell analysis and retrieval

Lab Chip. 2015 Feb 7;15(3):637-41. doi: 10.1039/c4lc00884g.

Abstract

We developed a micropatterned photodegradable hydrogel array integrated with reconfigurable microfluidics to enable cell secretion analysis and cell retrieval at the single-cell level. The activity of protease molecules secreted from single cells was monitored using FRET peptides entrapped inside microfabricated compartments. Antibody-modified gel islands tethering cells to the surface could be degraded by UV exposure to release specific single cells of interest.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Antibodies / immunology
  • CD4 Antigens / immunology
  • Fluorescence Resonance Energy Transfer
  • Humans
  • Hydrogel, Polyethylene Glycol Dimethacrylate / chemistry*
  • Microfluidic Analytical Techniques / instrumentation*
  • Microfluidic Analytical Techniques / methods*
  • Particle Size
  • Peptides / chemistry
  • Photochemical Processes
  • Single-Cell Analysis / instrumentation*
  • Single-Cell Analysis / methods*
  • Surface Properties
  • U937 Cells

Substances

  • Antibodies
  • CD4 Antigens
  • Peptides
  • Hydrogel, Polyethylene Glycol Dimethacrylate