Abstract
An enriched fraction of an aqueous extract prepared from the aerial parts of Cyclopia genistoides Vent. yielded a new benzophenone di-C,O-glucoside, 3-C-β-d-glucopyranosyl-4-O-β-d-glucopyranosyliriflophenone (1), together with small quantities of a known benzophenone C-glucoside, 3-C-β-d-glucopyranosylmaclurin (2). The isolated compounds showed α-glucosidase inhibitory activity against an enzyme mixture extracted from rat intestinal acetone powder. Compound 2 exhibited significantly (p < 0.05) higher inhibitory activity (54%) than 1 (43%) at 200 μM. In vitro tests in several cell models showed that 1 and its 3-C-monoglucosylated derivative (3-C-β-d-glucopyranosyliriflophenone) were marginally effective (p ≥ 0.05) in increasing glucose uptake.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Algorithms
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Animals
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Benzophenones / chemistry
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Benzophenones / isolation & purification*
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Benzophenones / pharmacology*
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Cyclopia Plant / chemistry*
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Glucose / metabolism
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Glucosides / chemistry
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Glucosides / isolation & purification*
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Glucosides / pharmacology*
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Glycoside Hydrolase Inhibitors / chemistry
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Glycoside Hydrolase Inhibitors / isolation & purification*
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Glycoside Hydrolase Inhibitors / pharmacology*
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Male
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Molecular Structure
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Nuclear Magnetic Resonance, Biomolecular
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Plant Leaves / chemistry
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Rats
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South Africa
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alpha-Glucosidases / drug effects
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alpha-Glucosidases / metabolism*
Substances
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3-C-beta-D-glucopyranosyl-4-O-beta-D-glucopyranosyliriflophenone
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3-C-beta-D-glucopyranosylmaclurin
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Benzophenones
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Glucosides
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Glycoside Hydrolase Inhibitors
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alpha-Glucosidases
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Glucose