This study investigated the effects of intraspecimen variations in tissue mineral density(TMD) on the apparent-level stiffness of human trabecular bone. High-resolution finite element (FE) models were created for each of 12 human trabecular bone specimens,using both microcomputed tomography (lCT) and “gold-standard” synchrotron radiation lCT (SRlCT) data. Our results confirm that incorporating TMD spatial variation reduces the calculated apparent stiffness compared to homogeneous TMD models. This effect exists for both lCT- and SRlCT-based FE models, but is exaggerated in lCT based models. This study provides a direct comparison of lCT to SRlCT data and is thereby able to conclude that the influence of including TMD heterogeneity is overestimated in lCT-based models.