As one of the famous medicinal plants in China, the bark of Eucommia ulmoides is considered as the important traditional Chinese medicine. However, the constituents of Eucommia ulmoides wood isn't used effectively. Therefore, the molecules of wood extracts in Eucommia ulmoides are studied to further utilize the resources. The results show that the optimal extraction time period of ethanol/methanol extraction, petroleum ether/acetic ether extraction, and benzene/alcohol extraction are 3h, 5h, and 4h, respectively. Among sequential extractions, PABEEM extraction is the optimum extraction mode for the LRs is 22.95%. Its wood extracts included hexanedioic acid, bis(2-ethylhexyl) ester, 3,3,7,11-tetramethyltricyclo [5.4.0.0 (4,11)] undecan-1-ol, (all-E)- 2,6,10,15,19,23-hexamethyl- 2,6,10,14,18,22-tetracosahexaene, other rare drug and biomedical activities. And the wood extracts of Eucommia ulmoides is fit to extract 1,5-hexadien-3-yne, (all-E)-2,6,10,15,19,23-hexamethyl-2,6,10,14,18,22-tetracosahexaene.