Helicobacter pylori γ-glutamyl transpeptidase-induced Ca(2+) release via PLC-IP3 receptors in AGS cells

Can J Microbiol. 2014 Dec;60(12):865-8. doi: 10.1139/cjm-2014-0464.

Abstract

In our previous study, γ-glutamyl transpeptidase (GGT) isolated from Helicobacter pylori induced apoptosis of AGS cells. Here, we investigate Ca(2+) effects on GGT-induced apoptosis. The GGT transiently and significantly increased intracellular Ca(2+) concentration ([Ca(2+)]i) in AGS cells in a dose-dependent manner (P < 0.05). The GGT-induced Ca(2+) increase resulted from Ca(2+) influx and release through the phospholipase C - inositol 1,4,5-trisphosphate (PLC-IP3) pathway. The GGT-induced apoptosis was significantly reduced by treatment with U73122 (a PLC inhibitor) and xestospongin (an IP3 receptor antagonist) (P < 0.05). These results indicate that GGT could induce apoptosis of AGS cells by high levels of [Ca(2+)]i.

Keywords: Helicobacter pylori; apoptose; apoptosis; calcium; γ-glutamyl transpeptidase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis*
  • Calcium / metabolism*
  • Cell Line, Tumor
  • Enzyme Inhibitors / pharmacology
  • Estrenes / pharmacology
  • Helicobacter pylori / metabolism*
  • Humans
  • Inositol 1,4,5-Trisphosphate Receptors / antagonists & inhibitors
  • Inositol 1,4,5-Trisphosphate Receptors / metabolism*
  • Macrocyclic Compounds / pharmacology
  • Oxazoles / pharmacology
  • Pyrrolidinones / pharmacology
  • Recombinant Proteins / metabolism
  • Type C Phospholipases / antagonists & inhibitors
  • Type C Phospholipases / metabolism*
  • gamma-Glutamyltransferase / genetics
  • gamma-Glutamyltransferase / metabolism*

Substances

  • Enzyme Inhibitors
  • Estrenes
  • Inositol 1,4,5-Trisphosphate Receptors
  • Macrocyclic Compounds
  • Oxazoles
  • Pyrrolidinones
  • Recombinant Proteins
  • xestospongin C
  • 1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione
  • gamma-Glutamyltransferase
  • Type C Phospholipases
  • Calcium