Quantification of fluorescent reporters in plant cells

Methods Mol Biol. 2015:1242:123-31. doi: 10.1007/978-1-4939-1902-4_11.

Abstract

Fluorescent reporters are powerful tools for plant research. Many studies require accurate determination of fluorescence intensity and localization. Here, we describe protocols for the quantification of fluorescence intensity in plant cells from confocal laser scanning microscope images using semiautomated software and image analysis techniques.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / chemistry
  • Arabidopsis / cytology*
  • Fluorescent Dyes
  • Image Processing, Computer-Assisted / methods
  • Laser Scanning Cytometry / methods*
  • Microscopy, Fluorescence / methods
  • Plant Cells / chemistry*
  • Plant Roots / chemistry
  • Plant Roots / cytology
  • Software

Substances

  • Fluorescent Dyes