Long-term thiol monitoring in living cells using bioorthogonal chemistry

Chem Commun (Camb). 2015;51(2):388-90. doi: 10.1039/c4cc08396b.

Abstract

Intracellular thiols play vital roles in living systems, and their in situ monitoring is of great importance. Here, we report on a bioorthogonal chemistry based fluorescent probe, which is capable of monitoring intracellular thiols in living cells for up to 36 hours with an obvious blue-to-green fluorescence change.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Survival
  • Fluorescent Dyes / chemistry*
  • HeLa Cells
  • Humans
  • Naphthalimides / chemistry*
  • Optical Imaging
  • Sulfhydryl Compounds / analysis*

Substances

  • Fluorescent Dyes
  • Naphthalimides
  • Sulfhydryl Compounds