Generation of iPS Cells from Granulosa Cells

Methods Mol Biol. 2016:1357:451-64. doi: 10.1007/7651_2014_135.

Abstract

Various types of somatic cells can be reprogrammed to induced pluripotent stem (iPS) cells. Somatic stem cells may generate iPS cells more efficiently than do differentiated cells. We show that granulosa cells exhibit characteristic of somatic stem cells and can be reprogrammed to iPS cells more efficiently or with few factors. Here, we describe generation of mouse and pig iPS cells from granulosa cells with high efficiency.

Keywords: Granulosa cells; Induced pluripotent stem cells (iPSCs); Mouse; Pig; Reprogramming.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Culture Techniques / methods*
  • Cells, Cultured
  • Cellular Reprogramming Techniques / methods*
  • Cellular Reprogramming*
  • Cryopreservation / methods
  • Culture Media / pharmacology
  • Embryoid Bodies
  • Female
  • Fibroblasts / cytology
  • Genetic Vectors / genetics
  • Gonadotropins, Equine / pharmacology
  • Granulosa Cells / chemistry
  • Granulosa Cells / cytology*
  • Granulosa Cells / drug effects
  • HEK293 Cells
  • Humans
  • Induced Pluripotent Stem Cells / cytology*
  • Lamin Type A / analysis
  • Mice
  • Mice, Inbred C57BL
  • Recombinant Fusion Proteins / genetics
  • Retroviridae / genetics
  • Sus scrofa
  • Swine
  • Transcription Factors / genetics

Substances

  • Culture Media
  • Gonadotropins, Equine
  • Lamin Type A
  • Recombinant Fusion Proteins
  • Transcription Factors