Establishment of a New Ovarian Cancer Cell Line CA5171

Ying-Cheng Chiang; Wen-Fang Cheng; Ming-Cheng Chang; Tzu-Pin Lu; Kuan-Ting Kuo; Hsiu-Ping Lin; Chang-Yao Hsieh; Chi-An Chen

doi:10.1177/1933719114557893

Establishment of a New Ovarian Cancer Cell Line CA5171

Reprod Sci. 2015 Jun;22(6):725-34. doi: 10.1177/1933719114557893. Epub 2014 Nov 12.

Authors

Ying-Cheng Chiang¹, Wen-Fang Cheng², Ming-Cheng Chang³, Tzu-Pin Lu⁴, Kuan-Ting Kuo⁵, Hsiu-Ping Lin³, Chang-Yao Hsieh³, Chi-An Chen⁶

Affiliations

¹ Department of Obstetrics and Gynecology, College of Medicine, National Taiwan University, Taipei, Taiwan Graduate Institute of Clinical Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan.
² Department of Obstetrics and Gynecology, College of Medicine, National Taiwan University, Taipei, Taiwan Graduate Institute of Clinical Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan Graduate Institute of Oncology, College of Medicine, National Taiwan University, Taipei, Taiwan.
³ Department of Obstetrics and Gynecology, College of Medicine, National Taiwan University, Taipei, Taiwan.
⁴ Department of Public Health, Graduate Institute of Epidemiology and Preventive Medicine, National Taiwan University, Taipei, Taiwan.
⁵ Department of Pathology, National Taiwan University Hospital, Taipei, Taiwan.
⁶ Department of Obstetrics and Gynecology, College of Medicine, National Taiwan University, Taipei, Taiwan chianchen@ntu.edu.tw.

Abstract

A new cell line, CA5171, derived from a chemotherapy-naive, high-grade undifferentiated ovarian carcinoma was established and characterized. The CA5171 cells presented with cobblestone morphology and a doubling time of 24 hours. Gene mutation analysis showed that the cells belonged to the type II ovarian cancer pathway with mutations of PIK3CA, PTEN, and TP53. Single-nucleotide polymorphism array analysis showed no homozygous gene deletion; however, several loci of gene copy number gains were noted in chromosome 1, 2, 5, 9, 10, 12, 15, 16, 20, and X. The in vitro and in vivo experiments showed that the cells were sensitive to paclitaxel and doxorubicin, but resistant to cisplatin. The cells also presented epithelial-mesenchymal transition properties that may have been related to their invasion and migration potential. The CA5171 cells show the potential as a new cell line for studies on epithelial ovarian carcinoma.

Keywords: cell line; epithelial ovarian carcinoma; type II pathway.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antineoplastic Agents / pharmacology
Biomarkers, Tumor / genetics
Biomarkers, Tumor / metabolism
Carcinoma, Ovarian Epithelial
Cell Line, Tumor
Cell Movement
Cell Proliferation
Cell Shape
Cisplatin / pharmacology
Class I Phosphatidylinositol 3-Kinases
DNA Copy Number Variations
Doxorubicin / pharmacology
Drug Resistance, Neoplasm
Epithelial-Mesenchymal Transition
Female
Gene Expression Regulation, Neoplastic
Genetic Predisposition to Disease
Humans
Middle Aged
Mutation
Neoplasm Invasiveness
Neoplasms, Glandular and Epithelial* / drug therapy
Neoplasms, Glandular and Epithelial* / genetics
Neoplasms, Glandular and Epithelial* / metabolism
Neoplasms, Glandular and Epithelial* / pathology
Ovarian Neoplasms* / drug therapy
Ovarian Neoplasms* / genetics
Ovarian Neoplasms* / metabolism
Ovarian Neoplasms* / pathology
PTEN Phosphohydrolase / genetics
PTEN Phosphohydrolase / metabolism
Paclitaxel / pharmacology
Phenotype
Phosphatidylinositol 3-Kinases / genetics
Phosphatidylinositol 3-Kinases / metabolism
Polymorphism, Single Nucleotide
Tumor Suppressor Protein p53 / genetics
Tumor Suppressor Protein p53 / metabolism

Substances

Antineoplastic Agents
Biomarkers, Tumor
TP53 protein, human
Tumor Suppressor Protein p53
Doxorubicin
Phosphatidylinositol 3-Kinases
Class I Phosphatidylinositol 3-Kinases
PIK3CA protein, human
PTEN Phosphohydrolase
PTEN protein, human
Paclitaxel
Cisplatin