Abstract
Pif1 is a helicase involved in the maintenance of nuclear and mitochondrial genomes in eukaryotes. Here we report a new activity of Saccharomyces cerevisiae Pif1, annealing of complementary DNA strands. We identified preferred substrates for annealing as those that generate a duplex product with a single-stranded overhang relative to a blunt end duplex. Importantly, we show that Pif1 can anneal DNA in the presence of ATP and Mg(2+). Pif1-mediated annealing also occurs in the presence of single-stranded DNA binding proteins. Additionally, we show that partial duplex substrates with 3'-single-stranded overhangs such as those generated during double-strand break repair can be annealed by Pif1.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenosine Triphosphate / metabolism
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Base Sequence
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DNA Breaks, Double-Stranded
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DNA Helicases / metabolism*
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DNA Repair
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DNA, Complementary / chemistry*
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DNA, Complementary / genetics
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DNA, Complementary / metabolism*
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DNA, Fungal / chemistry*
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DNA, Fungal / genetics
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DNA, Fungal / metabolism*
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Kinetics
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Magnesium / metabolism
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Molecular Sequence Data
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Nucleic Acid Conformation
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / metabolism
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Saccharomyces cerevisiae Proteins / metabolism*
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Substrate Specificity
Substances
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DNA, Complementary
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DNA, Fungal
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Saccharomyces cerevisiae Proteins
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Adenosine Triphosphate
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PIF1 protein, S cerevisiae
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DNA Helicases
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Magnesium