Direct stochastic optical reconstruction microscopy (dSTORM)

Ulrike Endesfelder; Mike Heilemann

doi:10.1007/978-1-4939-2080-8_14

Direct stochastic optical reconstruction microscopy (dSTORM)

Methods Mol Biol. 2015:1251:263-76. doi: 10.1007/978-1-4939-2080-8_14.

Authors

Ulrike Endesfelder¹, Mike Heilemann

Affiliation

¹ Institute of Physical & Theoretical Chemistry, Goethe University Frankfurt am Main, Max-von-Laue-Street 7, Frankfurt, 60438, Germany.

PMID: 25391804
DOI: 10.1007/978-1-4939-2080-8_14

Abstract

Single-molecule localization-based super-resolution microscopy can be performed with regular, bright, and photostable organic fluorophores. We review a concept termed direct stochastic optical reconstruction microscopy (dSTORM), which operates conventional fluorophores as photoswitches and provides an optical resolution of ~20 nm. We introduce the principle of dSTORM, illustrate experimental schemes, and discuss approaches for data analysis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cytological Techniques / methods*
Fluorescent Dyes*
Image Processing, Computer-Assisted / methods*
Microscopy, Fluorescence / methods*
Molecular Imaging / methods*
Staining and Labeling

Substances

Fluorescent Dyes