Customized patterned substrates for highly versatile correlative light-scanning electron microscopy

Lorena Benedetti; Elisa Sogne; Simona Rodighiero; Davide Marchesi; Paolo Milani; Maura Francolini

doi:10.1038/srep07033

Customized patterned substrates for highly versatile correlative light-scanning electron microscopy

Sci Rep. 2014 Nov 13:4:7033. doi: 10.1038/srep07033.

Authors

Lorena Benedetti¹, Elisa Sogne², Simona Rodighiero³, Davide Marchesi³, Paolo Milani⁴, Maura Francolini¹

Affiliations

¹ 1] Fondazione Filarete for Biosciences and Innovation, Viale Ortles 22/4, 20139, Milan, Italy [2] Department of Medical Biotechnology and Translational Medicine, Università degli Studi di Milano, and National Research Council (CNR) Neuroscience Institute, Via Vanvitelli 32, 20129 Milan, Italy.
² 1] Fondazione Filarete for Biosciences and Innovation, Viale Ortles 22/4, 20139, Milan, Italy [2] Interdisciplinary Centre for Nanostructured Materials and Interfaces (CIMaINa), and Department of Physics, Università degli Studi di Milano, Via Celoria 16, 20133 Milano, Italy [3] European School of Molecular Medicine (SEMM), IFOM-IEO, Via Adamello 16, 20139 Milano, Italy.
³ Fondazione Filarete for Biosciences and Innovation, Viale Ortles 22/4, 20139, Milan, Italy.
⁴ 1] Fondazione Filarete for Biosciences and Innovation, Viale Ortles 22/4, 20139, Milan, Italy [2] Interdisciplinary Centre for Nanostructured Materials and Interfaces (CIMaINa), and Department of Physics, Università degli Studi di Milano, Via Celoria 16, 20133 Milano, Italy.

Abstract

Correlative light electron microscopy (CLEM) combines the advantages of light and electron microscopy, thus making it possible to follow dynamic events in living cells at nanometre resolution. Various CLEM approaches and devices have been developed, each of which has its own advantages and technical challenges. We here describe our customized patterned glass substrates, which improve the feasibility of correlative fluorescence/confocal and scanning electron microscopy.