Abstract
This study investigated the treatment effects of a new compound, strontium fructose 1, 6-diphosphate (FDP-Sr), in cyclophosphamide (CP)-induced oligozoospermia. FDP-Sr, with extra high-energy supply, could reverse male hypogonadism in the testis. Male Wistar rats were randomly divided into three groups: control group (vehicle treated), CP group and CP + FDP-Sr group. Both CP group and CP + FDP-Sr groups were orally administered CP (20 mg kg(-1) ) consecutively for the first 7 days to establish CP-induced testicular toxic models. Subsequently, CP group was given orally distilled water per day, whereas CP + FDP-Sr group was received FDP-Sr (200 mg kg(-1) ) for 49 days. Compared to the CP group, the FDP-Sr group showed significantly increased levels of serum testosterone, testis relative weights and epididymal sperm counts in rats. In addition, rats treated by FDP-Sr showed the recuperative activities of testicular marker enzymes and normalised levels of antioxidants in tissue. Testicular protection of FDP-Sr was further demonstrated by enhancing expression of P450scc, reducing ability of FAS/FASL and generating cytoprotection in the histopathological study. FDP-Sr appeared to possess an ability to attenuate CP-induced reproduction toxicity via the activation of antioxidants and steroidogenesis enzymes, and alleviate oligozoospermia via inhibition of testicular apoptosis by FAS/FASL pathway.
Keywords:
Cyclophosphamide; oligozoospermia; strontium fructose 1, 6-diphosphate.
© 2014 Blackwell Verlag GmbH.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antineoplastic Agents, Alkylating / toxicity
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Antioxidants / pharmacology*
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Apoptosis / drug effects*
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Apoptosis / genetics
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Cyclophosphamide / toxicity
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Disease Models, Animal
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Fas Ligand Protein / drug effects
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Fas Ligand Protein / genetics
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Fas Ligand Protein / metabolism
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Fructosediphosphates / pharmacology*
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Glutathione / drug effects
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Glutathione / metabolism
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Glutathione Peroxidase / drug effects
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Glutathione Peroxidase / metabolism
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L-Iditol 2-Dehydrogenase / drug effects
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L-Iditol 2-Dehydrogenase / metabolism
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L-Lactate Dehydrogenase / drug effects
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L-Lactate Dehydrogenase / metabolism
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Lipid Peroxides / metabolism
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Male
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Malondialdehyde / metabolism
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Oligospermia / chemically induced
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Oligospermia / metabolism*
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Organ Size / drug effects
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Rats
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Rats, Wistar
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Reverse Transcriptase Polymerase Chain Reaction
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Sperm Count
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Sperm Retrieval
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Superoxide Dismutase / drug effects
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Superoxide Dismutase / metabolism
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Testis / anatomy & histology
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Testis / drug effects*
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Testosterone / blood
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fas Receptor / drug effects
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fas Receptor / genetics
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fas Receptor / metabolism
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gamma-Glutamyltransferase / drug effects
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gamma-Glutamyltransferase / metabolism
Substances
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Antineoplastic Agents, Alkylating
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Antioxidants
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Fas Ligand Protein
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Fas protein, rat
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Faslg protein, rat
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Fructosediphosphates
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Lipid Peroxides
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fas Receptor
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Testosterone
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Malondialdehyde
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Cyclophosphamide
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L-Iditol 2-Dehydrogenase
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L-Lactate Dehydrogenase
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Glutathione Peroxidase
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Superoxide Dismutase
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gamma-Glutamyltransferase
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Glutathione
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fructose-1,6-diphosphate