Production of a low calorie mandarin juice by enzymatic conversion of constituent sugars to oligosaccharides and prevention of insoluble glucan formation

Biotechnol Lett. 2015 Mar;37(3):711-6. doi: 10.1007/s10529-014-1723-y. Epub 2014 Nov 9.

Abstract

Over 99% of sucrose in mandarin juice (57.1 g/l in original juice to 428.4 g/l in concentrated juice) was enzymatically converted to glucooligosaccharides using 3 U dextransucrase/ml prepared from Leuconostoc mesenteroides at 28 °C. The oligosaccharide synthesis yields were 51 and 47% for the original and the concentrated mandarin juice, respectively. The degree of polymerization of oligosaccharides in the enzyme-modified juice was 2-7. Calories in the original and modified mandarin juice were 433 and 301 kcal/l (30.5% reduction). Compared with the original juice, the enzyme-modified juice showed 82% decrease of insoluble glucan formation by mutansucrase from Streptococcus mutans. A sensory evaluation of the juices revealed that the original and modified mandarin juices had sweetness values of 4.5 and 4.9 and the same values for overall acceptability.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Beverages*
  • Calorimetry
  • Food Handling / methods*
  • Glucosyltransferases / metabolism*
  • Leuconostoc / enzymology
  • Oligosaccharides / metabolism*
  • Streptococcus mutans / enzymology
  • Sucrase / metabolism*
  • Sucrose / metabolism*
  • Temperature

Substances

  • Oligosaccharides
  • Sucrose
  • Glucosyltransferases
  • dextransucrase
  • Sucrase