Hydrogel design of experiments methodology to optimize hydrogel for iPSC-NPC culture

Adv Healthc Mater. 2015 Mar 11;4(4):534-9. doi: 10.1002/adhm.201400410. Epub 2014 Nov 5.

Abstract

Bioactive signals can be incorporated in hydrogels to direct encapsulated cell behavior. Design of experiments methodology methodically varies the signals systematically to determine the individual and combinatorial effects of each factor on cell activity. Using this approach enables the optimization of three ligands concentrations (RGD, YIGSR, IKVAV) for the survival and differentiation of neural progenitor cells.

Keywords: design of experiments; hyaluronic acid; hydrogel; induced pluripotent stem cells; neurons.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Cell Adhesion / drug effects
  • Cell Culture Techniques / methods*
  • Humans
  • Hyaluronic Acid / pharmacology
  • Hydrogel, Polyethylene Glycol Dimethacrylate / pharmacology*
  • Induced Pluripotent Stem Cells / cytology*
  • Induced Pluripotent Stem Cells / drug effects
  • Magnetic Resonance Spectroscopy
  • Molecular Sequence Data
  • Neural Stem Cells / cytology*
  • Neural Stem Cells / drug effects
  • Peptides / chemistry
  • Peptides / pharmacology

Substances

  • Peptides
  • Hydrogel, Polyethylene Glycol Dimethacrylate
  • Hyaluronic Acid