SNP genotyping using KASPar assays

Scott M Smith; Peter J Maughan

doi:10.1007/978-1-4939-1966-6_18

SNP genotyping using KASPar assays

Methods Mol Biol. 2015:1245:243-56. doi: 10.1007/978-1-4939-1966-6_18.

Authors

Scott M Smith¹, Peter J Maughan

Affiliation

¹ Department of Plant and Microbial Biology, North Carolina State University, 600 Laureate Way, Kannapolis, 28081, NC, smsmit30@ncsu.edu.

PMID: 25373762
DOI: 10.1007/978-1-4939-1966-6_18

Abstract

In a separate chapter we describe a simple method for single nucleotide polymorphism (SNP) discovery using genomic reduction. Here we describe a scalable and cost-effective SNP genotyping method based on KBioscience's competitive allele-specific PCR amplification of target sequences and endpoint fluorescence genotyping (KASPar™) using a FRET capable plate reader or Fluidigm's dynamic array high-throughput platform.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alleles*
DNA Primers / metabolism
Fluorescence Resonance Energy Transfer
Genotyping Techniques / methods*
Polymerase Chain Reaction / methods*
Polymorphism, Single Nucleotide / genetics*

Substances

DNA Primers