Isolation and characterization of marine Brevibacillus sp. S-1 collected from South China Sea and a novel antitumor peptide produced by the strain

Lanhong Zheng; Yao Yi; Jia Liu; Xiukun Lin; Kangli Yang; Mei Lv; Xinwen Zhou; Jianhua Hao; Junzhong Liu; Yuan Zheng; Mi Sun

doi:10.1371/journal.pone.0111270

Isolation and characterization of marine Brevibacillus sp. S-1 collected from South China Sea and a novel antitumor peptide produced by the strain

PLoS One. 2014 Nov 5;9(11):e111270. doi: 10.1371/journal.pone.0111270. eCollection 2014.

Authors

Lanhong Zheng¹, Yao Yi¹, Jia Liu², Xiukun Lin³, Kangli Yang⁴, Mei Lv⁴, Xinwen Zhou⁵, Jianhua Hao¹, Junzhong Liu¹, Yuan Zheng¹, Mi Sun¹

Affiliations

¹ Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, PR China.
² Qingdao University, Qingdao, PR China.
³ Department of Pharmacology, Capital Medical University, Beijing, PR China.
⁴ Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, PR China; Qingdao University of Science & Technology, Qingdao, PR China.
⁵ Institutes of Biomedical Sciences, Fudan University, Shanghai, PR China.

Abstract

A Gram-positive, rod-shaped bacterium, designated as S-1, was isolated from a marine sediment sample collected from South China Sea. Phylogenetic analysis based on 16S rRNA gene sequence showed that S-1 belongs to the genus Brevibacillus. A novel cytotoxic peptide was isolated from the fermentation broth of the marine-derived bacterium Brevibacillus sp. S-1, using ion-exchange chromatography and reverse-phase HPLC chromatography. The molecular weight of this peptide was determined as 1570 Da by MALDI-TOF mass spectrometry, and its structure was proposed as a cyclic peptide elucidated by MALDI-TOF/TOF mass spectrometry and de novo sequencing. 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay showed that this peptide exhibited cytotoxicity against BEL-7402 human hepatocellular carcinoma cells, RKO human colon carcinoma cells, A549 human lung carcinoma cells, U251 human glioma cells and MCF-7 human breast carcinoma cells. Additionally, SBP exhibited low cytotoxicity against HFL1 human normal fibroblast lung cells. The result suggested that the cytotoxic effect of the peptide is specific to tumor cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antineoplastic Agents / chemistry
Antineoplastic Agents / metabolism
Antineoplastic Agents / pharmacology*
Brevibacillus / classification
Brevibacillus / genetics
Brevibacillus / metabolism*
Cell Line, Tumor
Cell Survival / drug effects
Fermentation
Humans
Molecular Weight
Peptide Biosynthesis*
Peptides / chemistry
Peptides / metabolism
Peptides / pharmacology*
Phenotype
Phylogeny
RNA, Bacterial
RNA, Ribosomal, 16S
Seawater / microbiology*

Substances

Antineoplastic Agents
Peptides
RNA, Bacterial
RNA, Ribosomal, 16S

Grants and funding

This work was supported by Special Scientific Research Funds for Central Non-profit Institutes, Chinese Academy of Fishery Sciences (2013B01YQ01), Science and Technology Development Plans of Shandong Province (2014GGF01060), and Special Scientific Research Funds for Central Non-profit Institutes, Yellow Sea Fisheries Research Institutes (20603022013017 and 20603022012013). This work was also partly supported by 863 High Technology Project (2014AA093503). The authors are grateful to all members of the laboratory for their continuous technical advice and helpful discussion. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.