Conjugative reporter system for the use in Bacillus licheniformis and closely related Bacilli

R Hertel; S Volland; H Liesegang

doi:10.1111/lam.12352

Conjugative reporter system for the use in Bacillus licheniformis and closely related Bacilli

Lett Appl Microbiol. 2015 Feb;60(2):162-167. doi: 10.1111/lam.12352. Epub 2014 Dec 15.

Authors

R Hertel¹, S Volland¹, H Liesegang¹

Affiliation

¹ Department of Genomic and Applied Microbiology, Institute of Microbiology and Genetics, Georg-August University of Göttingen, Göttingen, Germany.

PMID: 25363901
DOI: 10.1111/lam.12352

Abstract

Bacillus wild-type strains are genetically difficult to manipulate, and thus, the options for rational strain investigation and design are limited. Here, we present a set of small conjugative shuttle vectors for the use in Bacillus licheniformis and related, genetically difficult accessible wild-type strains. The vector set comprises the modular general-purpose vector pV2 and its derivatives pV3SDlacZ and pV3lacZ. The pV3 vectors are designed for the investigation of transcriptional and translational activities of regulatory regions like promoters and ribosomal binding sites (RBS). The vector set has been tested for investigating gene regulation under aerobic and anaerobic conditions.

Keywords: Bacillus licheniformis DSM13; Firmicutes; Shuttle vector; beta-galactosidase; lacZ; pV2; pV3SDlacZ; pV3lacZ.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacillus / genetics*
Conjugation, Genetic*
Escherichia coli / genetics
Genes, Reporter
Genetic Vectors*
Lac Operon
Plasmids
Promoter Regions, Genetic