Computationally assisted engineering of protein cages

Maziar S Ardejani; Brendan P Orner

doi:10.1007/978-1-4939-2131-7_5

Computationally assisted engineering of protein cages

Methods Mol Biol. 2015:1252:51-9. doi: 10.1007/978-1-4939-2131-7_5.

Authors

Maziar S Ardejani¹, Brendan P Orner

Affiliation

¹ Department of Chemistry, School of Natural and Mathematical Sciences, King's College London, London, UK.

PMID: 25358772
DOI: 10.1007/978-1-4939-2131-7_5

Abstract

A hybrid computational method incorporating topographic analysis of protein surfaces and free-energy calculations of protein-protein interactions in protein nanocages is described. This design strategy can be used to engineer protein cages for enhanced structural stability and assembly.

MeSH terms

Computer-Aided Design*
Ferritins / chemistry
Ferritins / genetics
Models, Molecular
Mutagenesis, Site-Directed
Protein Binding
Protein Conformation
Protein Engineering / methods*
Protein Multimerization
Proteins / chemistry*
Proteins / genetics

Substances

Proteins
Ferritins