Synthesis and LC-MS/MS analysis of desmosine-CH2, a potential internal standard for the degraded elastin biomarker desmosine

Org Biomol Chem. 2014 Dec 28;12(48):9887-94. doi: 10.1039/c4ob01438c.

Abstract

Desmosine-CH2, an analog of the elastic tissue degradation biomarker desmosine, can be regarded as a potential internal standard for precise quantification of desmosines by LC-MS/MS. In this study, the chemical synthesis of desmosine-CH2 was completed in 22% overall yield in five steps. The LC-MS/MS analysis of desmosine-CH2 was also achieved.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomarkers / analysis
  • Chromatography, Liquid
  • Desmosine / analysis*
  • Desmosine / chemical synthesis
  • Elastin / chemistry*
  • Molecular Conformation
  • Tandem Mass Spectrometry

Substances

  • Biomarkers
  • Desmosine
  • Elastin