Complete genome sequence of bacteriophage vB_YenP_AP5 which infects Yersinia enterocolitica of serotype O:3

Virol J. 2014 Oct 28:11:188. doi: 10.1186/1743-422X-11-188.

Abstract

Background: Bacteriophage vB_YenP_AP5 is a lytic bacteriophage capable of infecting Yersinia enterocolitica strains of serotype O:3, an epidemiologically significant serotype within this bacterial species that causes yersiniosis in humans. This work describes the complete genome sequence of this phage.

Results: The genome consists of linear double-stranded DNA of 38,646 bp, with direct terminal repeats of 235 bp in length, and a GC content of 50.7%. There are 45 open reading frames which occupy 89.9% of the genome. Most of the proteins encoded by this virus exhibit sequence similarity to Yersinia phage φYeO3-12 and Salmonella phage φSG-JL2 proteins.

Conclusions: Genomic and morphological analyses place the bacteriophage vB_YenP_AP5 in the T7likevirus genus of the subfamily Autographivirinae within the family Podoviridae.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteriophages / classification
  • Bacteriophages / genetics*
  • Bacteriophages / isolation & purification
  • Bacteriophages / physiology
  • Base Sequence
  • Genome, Viral*
  • Host Specificity
  • Humans
  • Molecular Sequence Data
  • Open Reading Frames
  • Podoviridae / classification
  • Podoviridae / genetics*
  • Podoviridae / isolation & purification
  • Serotyping
  • Sewage / virology*
  • Yersinia enterocolitica / classification
  • Yersinia enterocolitica / isolation & purification
  • Yersinia enterocolitica / virology*

Substances

  • Sewage