Objective: To investigate the effects of different culture conditions on the main chemical compositions of Anoectochilus roxburghii, so as to determine the optimum culture conditions and provide theoretical support for large-scale production of Anoectochilus roxburghii.
Methods: The light qualities, photoperiods and induction periods were changed to regulate the main chemical compositions of Anoectochilus roxburghii.
Results: The contents of total flavonoids, quercetin, isorhamnetin and kaempferol in blue light were higher than that in yellow light, the worst was under red light. There was the highest total flavonoids, kaempferol and isorhamnetin content in photoperiod of 14 h/d. After one month inoculation, the total flavonoids, quercetin, isorhamnetin and kaempferol contents were the highest.
Conclusion: The results show that the optimum culture condition is: inducted 15 days with blue light inoculated one month later at the photoperiod of 14 h/d.