H5N1 Avian Influenza Pre-pandemic Vaccine Strains in China

Biomed Environ Sci. 2014 Oct;27(10):763-9. doi: 10.3967/bes2014.111.

Abstract

Objective: To prepare the 4 candidate vaccine strains of H5N1 avian influenza virus isolated in China.

Methods: Recombinant viruses were rescued using reverse genetics. Neuraminidase (NA) and hemagglutinin (HA) segments of the A/Xinjiang/1/2006, A/Guangxi/1/2009, A/Hubei/1/2010, and A/Guangdong/1/2011 viruses were amplified by RT-PCR. Multibasic amino acid cleavage site of HA was removed and ligated into the pCIpolI vector for virus rescue. The recombinant viruses were evaluated by trypsin dependent assays. Their embryonate survival and antigenicity were compared with those of the respective wild-type viruses.

Results: The 4 recombinant viruses showed similar antigenicity compared with wild-type viruses, chicken embryo survival and trypsin-dependent characteristics.

Conclusion: The 4 recombinant viruses rescued using reverse genetics meet the criteria for classification of low pathogenic avian influenza strains, thus supporting the use of them for the development of seeds and production of pre-pandemic vaccines.

Keywords: H5N1; Influenza; Pre-pandemic; Vaccine strains.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chick Embryo
  • Chickens
  • China
  • Hemagglutinin Glycoproteins, Influenza Virus / genetics
  • Hemagglutinin Glycoproteins, Influenza Virus / metabolism
  • Influenza A Virus, H5N1 Subtype / immunology*
  • Influenza Vaccines / immunology*
  • Influenza in Birds / prevention & control*
  • Influenza in Birds / virology*
  • Neuraminidase / genetics
  • Neuraminidase / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Vaccines, Synthetic / immunology

Substances

  • Hemagglutinin Glycoproteins, Influenza Virus
  • Influenza Vaccines
  • Vaccines, Synthetic
  • Neuraminidase