Aptamer-mediated 'turn-off/turn-on' nanozyme activity of gold nanoparticles for kanamycin detection

Tarun Kumar Sharma; Rajesh Ramanathan; Pabudi Weerathunge; Mahsa Mohammadtaheri; Hemant Kumar Daima; Ravi Shukla; Vipul Bansal

doi:10.1039/c4cc07275h

Aptamer-mediated 'turn-off/turn-on' nanozyme activity of gold nanoparticles for kanamycin detection

Chem Commun (Camb). 2014 Dec 28;50(100):15856-9. doi: 10.1039/c4cc07275h.

Authors

Tarun Kumar Sharma¹, Rajesh Ramanathan, Pabudi Weerathunge, Mahsa Mohammadtaheri, Hemant Kumar Daima, Ravi Shukla, Vipul Bansal

Affiliation

¹ Ian Potter NanoBioSensing Facility, NanoBiotechnology Research Laboratory, School of Applied Science, RMIT University, GPO Box 2476V, Melbourne VIC 3001, Australia. vipul.bansal@rmit.edu.au.

PMID: 25331713
DOI: 10.1039/c4cc07275h

Abstract

A new ultrafast and highly sensitive 'turn-off/turn-on' biosensing approach that combines the intrinsic peroxidase-like activity of gold nanoparticles (GNPs) with the high affinity and specificity of a ssDNA aptamer is presented for the efficient detection of a model small molecule kanamycin.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aptamers, Nucleotide / chemistry*
Biosensing Techniques*
DNA, Single-Stranded / chemistry
Gold / chemistry*
Kanamycin / analysis*
Metal Nanoparticles / chemistry*
Peroxidase / chemistry
Peroxidase / metabolism

Substances

Aptamers, Nucleotide
DNA, Single-Stranded
Kanamycin
Gold
Peroxidase