Abstract
In this method paper we describe the spectral phasor analysis applied to Laurdan emission for the assessment of the fluidity of different membranes in live cells. We first introduce the general context and then we show how to obtain the spectral phasor from data acquired using a commercial microscope.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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2-Naphthylamine / analogs & derivatives*
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Animals
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Cell Membrane / chemistry*
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Cell Membrane / metabolism
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Cholera Toxin / chemistry
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HEK293 Cells
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Humans
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Laurates*
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Membrane Lipids / chemistry
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Mice
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Molecular Biology / methods
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NIH 3T3 Cells
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Spectrometry, Fluorescence / methods*
Substances
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Laurates
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Membrane Lipids
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Cholera Toxin
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2-Naphthylamine
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laurdan