The Laurdan spectral phasor method to explore membrane micro-heterogeneity and lipid domains in live cells

Methods Mol Biol. 2015:1232:273-90. doi: 10.1007/978-1-4939-1752-5_19.

Abstract

In this method paper we describe the spectral phasor analysis applied to Laurdan emission for the assessment of the fluidity of different membranes in live cells. We first introduce the general context and then we show how to obtain the spectral phasor from data acquired using a commercial microscope.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • 2-Naphthylamine / analogs & derivatives*
  • Animals
  • Cell Membrane / chemistry*
  • Cell Membrane / metabolism
  • Cholera Toxin / chemistry
  • HEK293 Cells
  • Humans
  • Laurates*
  • Membrane Lipids / chemistry
  • Mice
  • Molecular Biology / methods
  • NIH 3T3 Cells
  • Spectrometry, Fluorescence / methods*

Substances

  • Laurates
  • Membrane Lipids
  • Cholera Toxin
  • 2-Naphthylamine
  • laurdan