Genetic inactivation of European sea bass (Dicentrarchus labrax L.) eggs using UV-irradiation: observations and perspectives

Julie Colléter; David J Penman; Stéphane Lallement; Christian Fauvel; Tanja Hanebrekke; Renate D Osvik; Hans C Eilertsen; Helena D'Cotta; Béatrice Chatain; Stefano Peruzzi

doi:10.1371/journal.pone.0109572

Genetic inactivation of European sea bass (Dicentrarchus labrax L.) eggs using UV-irradiation: observations and perspectives

PLoS One. 2014 Oct 20;9(10):e109572. doi: 10.1371/journal.pone.0109572. eCollection 2014.

Authors

Affiliations

¹ Cirad, Persyst, UMR Intrepid, Campus International de Baillarguet, Montpellier, France; Ifremer, Laboratoire de Recherche Piscicole en Méditerranée, Station Expérimentale d'Aquaculture, Palavas Les Flots, France.
² University of Stirling, Institute of Aquaculture, Stirling, Scotland.
³ Ifremer, Laboratoire de Recherche Piscicole en Méditerranée, Station Expérimentale d'Aquaculture, Palavas Les Flots, France.
⁴ Department of Arctic and Marine Biology, Faculty of Biosciences, Fisheries and Economics, University of Tromsø, Tromsø, Norway.
⁵ Cirad, Persyst, UMR Intrepid, Campus International de Baillarguet, Montpellier, France.

Abstract

Androgenesis is a form of uniparental reproduction leading to progenies inheriting only the paternal set of chromosomes. It has been achieved with variable success in a number of freshwater species and can be attained by artificial fertilization of genetically inactivated eggs following exposure to gamma (γ), X-ray or UV irradiation (haploid androgenesis) and by restoration of diploidy by suppression of mitosis using a pressure or thermal shock. The conditions for the genetic inactivation of the maternal genome in the European sea bass (Dicentrarchus labrax L.) were explored using different combinations of UV irradiation levels and durations. UV treatments significantly affected embryo survival and generated a wide range of developmental abnormalities. Despite the wide range of UV doses tested (from 7.2 to 720 mJ x cm(-2)), only one dose (60 mJ x cm(-2) x min(-1) with 1 min irradiation) resulted in a small percentage (14%) of haploid larvae at hatching in the initial trials as verified by flow cytometry. Microsatellite marker analyses of three further batches of larvae produced by using this UV treatment showed a majority of larvae with variable levels of paternal and maternal contributions and only one larva displaying pure paternal inheritance. The results are discussed also in the context of an assessment of the UV-absorbance characteristics of egg extracts in this species that revealed the presence of gadusol, a compound structurally related to mycosporine-like amino acids (MAAs) with known UV-screening properties.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bass / embryology
Bass / genetics*
Diploidy
Embryo, Nonmammalian / physiology
Embryo, Nonmammalian / radiation effects
Gene Silencing / radiation effects*
Larva / radiation effects
Microsatellite Repeats / genetics
Ovum / metabolism*
Ovum / radiation effects*
Ploidies
Survival Analysis
Ultraviolet Rays*

Grants and funding

Julie Colléter’s doctoral study was partly funded under the EU Seventh Framework Programme by the AQUAEXCEL project N° 262336: AQUAculture infrastructures for EXCELlence in European fish research. The views expressed in this work are the sole responsibility of the authors and do not necessarily reflect the views of the European Commission. Additional financial support was provided by the (Unité Mixte de recherche - Intensification raisonnée) UMR Intrepid (Cirad-Ifremer) and the Department of Arctic and Marine Biology (University of Tromsø). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.