Ultrasound is emerging as a powerful tool for developing biomaterials for regenerative medicine. Ultrasound technologies are finding wide-ranging, innovative applications for controlling the fabrication of bioengineered scaffolds, as well as for imaging and quantitatively monitoring the properties of engineered constructs both during fabrication processes and post-implantation. This review provides an overview of the biomedical applications of ultrasound for imaging and therapy, a tutorial of the physical mechanisms through which ultrasound can interact with biomaterials, and examples of how ultrasound technologies are being developed and applied for biomaterials fabrication processes, non-invasive imaging, and quantitative characterization of bioengineered scaffolds in vitro and in vivo.