Abstract
We report the discovery of a fluorogenic dye, N(1),N(3)-di(2-aminidonaphthalen-6-yl) propane-1,3-diamine, MitoBlue, which selectively stains functional mitochondria while displaying low toxicity, bright blue emission, and high resistance to photobleaching. Additionally, we show that a biotin-labeled MitoBlue derivative can be used as a handle for the delivery of streptavidin-tagged species to the mitochondria.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
2-Naphthylamine / analogs & derivatives*
-
2-Naphthylamine / chemical synthesis
-
2-Naphthylamine / chemistry
-
Amidines / chemical synthesis
-
Amidines / chemistry*
-
Animals
-
Biotin / chemistry
-
Chickens
-
Chlorocebus aethiops
-
Embryo, Nonmammalian
-
Fibroblasts / physiology
-
Fibroblasts / ultrastructure*
-
Fluorescent Dyes / chemical synthesis
-
Fluorescent Dyes / chemistry*
-
Microscopy, Fluorescence
-
Mitochondria / physiology
-
Mitochondria / ultrastructure*
-
Staining and Labeling
-
Streptavidin / chemistry
-
Vero Cells
Substances
-
Amidines
-
Fluorescent Dyes
-
MitoBlue
-
Biotin
-
Streptavidin
-
2-Naphthylamine