MitoBlue: a nontoxic and photostable blue-emitting dye that selectively labels functional mitochondria

ACS Chem Biol. 2014 Dec 19;9(12):2742-7. doi: 10.1021/cb500552f. Epub 2014 Oct 21.

Abstract

We report the discovery of a fluorogenic dye, N(1),N(3)-di(2-aminidonaphthalen-6-yl) propane-1,3-diamine, MitoBlue, which selectively stains functional mitochondria while displaying low toxicity, bright blue emission, and high resistance to photobleaching. Additionally, we show that a biotin-labeled MitoBlue derivative can be used as a handle for the delivery of streptavidin-tagged species to the mitochondria.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 2-Naphthylamine / analogs & derivatives*
  • 2-Naphthylamine / chemical synthesis
  • 2-Naphthylamine / chemistry
  • Amidines / chemical synthesis
  • Amidines / chemistry*
  • Animals
  • Biotin / chemistry
  • Chickens
  • Chlorocebus aethiops
  • Embryo, Nonmammalian
  • Fibroblasts / physiology
  • Fibroblasts / ultrastructure*
  • Fluorescent Dyes / chemical synthesis
  • Fluorescent Dyes / chemistry*
  • Microscopy, Fluorescence
  • Mitochondria / physiology
  • Mitochondria / ultrastructure*
  • Staining and Labeling
  • Streptavidin / chemistry
  • Vero Cells

Substances

  • Amidines
  • Fluorescent Dyes
  • MitoBlue
  • Biotin
  • Streptavidin
  • 2-Naphthylamine