Objectives: This study was designed to adapt a previously developed in vitro microcosm biofilm model to create carries-affected dentin (CAD) and establish conditions for using the model in bonding studies.
Materials and methods: Biofilms were originated from human saliva and grown on dentin discs for 0 (sound dentin), 3, 5, 7, 14, or 21 days under intermittent cariogenic condition (n = 10). At each time point, composite cylinders were bonded to the dentin using self-etch adhesive (Clearfil SE Bond). The response variables were integrated mineral loss (ΔS), lesion depth (LD), shear bond strength (SBS), and failure mode. Data were statistically analyzed (α = 0.05). Bonded interfaces were analyzed by scanning electron microscopy (SEM), and dentin surfaces characterized by infrared spectroscopy (Fourier transform infrared spectroscopy, FTIR).
Results: Lower ΔS was found for sound dentin than for CAD in all experimental groups, except for the group under cariogenic challenge for 3 days. The SBS to CAD was significantly lower than control for all cariogenic challenge times. Adhesive failures were predominant in all groups. ΔS and LD had a significant negative correlation with SBS. A significant exponential decay in SBS was associated with increased ΔS values. CAD had lower mineral and amide I content and an irregular hybridization interface compared to sound dentin.
Conclusions: The microcosm biofilm model was able to artificially induce CAD, which imposed challenge to the bonding of the polymeric adhesive material.
Clinical relevance: Presence of CAD might interfere with the bonding of polymeric materials. The microcosm biofilm model proposed could be useful for preclinical dentin bonding studies.