Abstract
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been recently introduced to many diagnostic microbiological laboratories. Besides the identification of bacteria and fungi, that technique provides a potentially useful tool for the detection of antimicrobial resistance, especially of that conferred by β-lactamases. Here, we describe an assay allowing a detection of meropenem hydrolysis in clinical isolates of Enterobacteriaceae, Pseudomonas spp., and Acinetobacter baumannii using MALDI-TOF MS. This method is able to confirm carbapenemases within 3 h. The results are important for proper and fast intervention to limit the spread of carbapenemase-producing bacteria and provide information for appropriate initial therapy of the infections caused by these microbes.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acinetobacter Infections / diagnosis
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Acinetobacter Infections / drug therapy
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Acinetobacter Infections / microbiology
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Acinetobacter baumannii / drug effects
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Acinetobacter baumannii / enzymology*
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Acinetobacter baumannii / genetics
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Acinetobacter baumannii / isolation & purification
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Anti-Bacterial Agents / pharmacology
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism*
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Biological Assay*
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Gene Expression
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Humans
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Hydrolysis
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Meropenem
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Pseudomonas / drug effects
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Pseudomonas / enzymology*
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Pseudomonas / genetics
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Pseudomonas / isolation & purification
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Pseudomonas Infections / diagnosis
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Pseudomonas Infections / drug therapy
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Pseudomonas Infections / microbiology
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Thienamycins / pharmacology
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beta-Lactam Resistance / genetics*
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beta-Lactamases / genetics
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beta-Lactamases / metabolism*
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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Thienamycins
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beta-Lactamases
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carbapenemase
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Meropenem