Abstract
The 26S proteasome is a cellular proteolytic complex containing 19S regulatory particles and the 20S core proteasome. It was reported that the small molecule b-AP15 targets the proteasome by inhibiting deubiquitination of the 19S regulatory particles of the proteasome complex. An investigation of b-AP15 on the 20S proteasome core suggested that this compound can also inhibit the 20S proteasome with a potency equivalent to that found to inhibit the 19S regulatory particles.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism
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HeLa Cells / drug effects
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Humans
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Inhibitory Concentration 50
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Piperidones / chemistry
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Piperidones / pharmacology*
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Protease Inhibitors / pharmacology*
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Proteasome Endopeptidase Complex / metabolism*
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Ubiquitin / genetics
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Ubiquitin / metabolism
Substances
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3,5-bis((4-nitrophenyl)methylidene)-1-prop-2-enoylpiperidin-4-one
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Piperidones
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Protease Inhibitors
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Ubiquitin
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Green Fluorescent Proteins
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Proteasome Endopeptidase Complex
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26S proteasome non-ATPase regulatory subunit 13