An ESR and DFT study of hydration of the 2'-deoxyuridine-5-yl radical: a possible hydroxyl radical intermediate

Chem Commun (Camb). 2014 Dec 4;50(93):14605-8. doi: 10.1039/c4cc07089e.

Abstract

The mechanism of radiation-induced frank strand break formation in irradiated 5-bromo-2'-deoxyuridine (BrdU)-labelled DNA is still unclear despite the proven radiosensitizing properties of BrdU. Combination of ESR spectroscopy and quantum chemical modelling points to a simple reaction between the uridine-5-yl radical and water molecules that produces the genotoxic hydroxyl radical.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Deoxyuridine / chemistry*
  • Electron Spin Resonance Spectroscopy
  • Free Radicals / chemistry*
  • Hydroxyl Radical / chemistry*
  • Molecular Structure
  • Quantum Theory*
  • Thermodynamics
  • Water / chemistry

Substances

  • Free Radicals
  • Water
  • Hydroxyl Radical
  • Deoxyuridine