Aggregation of whey protein hydrolysate using Alcalase 2.4 L

PLoS One. 2014 Oct 7;9(10):e109439. doi: 10.1371/journal.pone.0109439. eCollection 2014.

Abstract

Here, we describe peptide aggregation, which is also known as enzymatic protein resynthesis. Whey protein hydrolysate (WPH) is the starting material for assembling peptides. Analyses of the involved amino acids, intrinsic fluorescence, fluorescence phase diagram, secondary structure, turbidity, and surface hydrophobicity were performed to investigate the reaction process. The aggregation mechanism consists of two parts: 1) formation and 2) aggregation of the building blocks that form the ordered secondary β-sheet structure. Constructing the building blocks requires at least one intermediate state, which is formed after 0.5 hours. Non-synergistic changes in the secondary and tertiary structures then allow the intermediate state to emerge.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acids / chemistry
  • Hydrophobic and Hydrophilic Interactions
  • Peptides / chemistry*
  • Protein Aggregates*
  • Protein Hydrolysates / chemistry*
  • Protein Structure, Secondary
  • Protein Structure, Tertiary
  • Subtilisins / chemistry*
  • Whey Proteins / chemistry*

Substances

  • Amino Acids
  • Peptides
  • Protein Aggregates
  • Protein Hydrolysates
  • Whey Proteins
  • Subtilisins

Grants and funding

Funding was provided by the Youth Natural Science Fund of Heilongjiang Province, China (Project No: QC2010029), and the Science Fund of NEAU. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.