Crystallization and preliminary X-ray diffraction analysis of Lin1840, a putative β-glucosidase from Listeria innocua

Acta Crystallogr F Struct Biol Commun. 2014 Oct;70(Pt 10):1398-401. doi: 10.1107/S2053230X14018597. Epub 2014 Sep 25.

Abstract

Lin1840 is a putative β-glucosidase that is predicted to be involved in 1,2-β-glucan metabolism since the lin1839 gene encoding a 1,2-β-oligoglucan phosphorylase and the lin1840 gene are located in the same gene cluster. Here, Lin1840 was crystallized. The crystals of Lin1840 diffracted to beyond 1.8 Å resolution. The crystal belonged to space group I121, with unit-cell parameters a = 89.75, b = 95.10, c = 215.00 Å, α = 90.00, β = 96.34, γ = 90.00°.

Keywords: 1,2-β-glucan; β-glucosidase.

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / chemistry*
  • Crystallization
  • Crystallography, X-Ray
  • Listeria / enzymology*
  • Molecular Sequence Data
  • beta-Glucosidase / chemistry*

Substances

  • Bacterial Proteins
  • beta-Glucosidase