Peptidomics study of anthocyanin-rich juice of elderberry

Hanzhi Wu; Mitch C Johnson; Chi-Hua Lu; Kevin L Fritsche; Andrew L Thomas; Yongquan Lai; Zongwei Cai; C Michael Greenlief

doi:10.1016/j.talanta.2014.08.022

Peptidomics study of anthocyanin-rich juice of elderberry

Talanta. 2015 Jan:131:640-4. doi: 10.1016/j.talanta.2014.08.022. Epub 2014 Aug 27.

Authors

Hanzhi Wu¹, Mitch C Johnson², Chi-Hua Lu³, Kevin L Fritsche³, Andrew L Thomas⁴, Yongquan Lai⁵, Zongwei Cai⁶, C Michael Greenlief⁷

Affiliations

¹ Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong SAR, PR of China; Department of Chemistry, University of Missouri, Columbia, MO, USA; Center for Botanical Interaction Studies, University of Missouri, Columbia, MO, USA.
² Department of Chemistry, University of Missouri, Columbia, MO, USA; Center for Botanical Interaction Studies, University of Missouri, Columbia, MO, USA.
³ Center for Botanical Interaction Studies, University of Missouri, Columbia, MO, USA; Division of Animal Sciences, University of Missouri, Columbia, MO, USA.
⁴ Center for Botanical Interaction Studies, University of Missouri, Columbia, MO, USA; Southwest Research Center, University of Missouri, Columbia, MO, USA.
⁵ Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong SAR, PR of China.
⁶ Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong SAR, PR of China. Electronic address: greenliefm@missouri.edu.
⁷ Department of Chemistry, University of Missouri, Columbia, MO, USA; Center for Botanical Interaction Studies, University of Missouri, Columbia, MO, USA. Electronic address: zwcai@hkbu.edu.hk.

PMID: 25281152
DOI: 10.1016/j.talanta.2014.08.022

Abstract

Biologically active peptides play a role in plant signaling and defense. Elderberry juice is known to contain a variety of anthocyanin compounds, a sub-set of polyphenols, which are responsible for the deep purple color of the juice. In this paper, we describe a method utilizing solid phase extraction (SPE) to remove anthocyanins from peptides. Liquid chromatography coupled with tandem mass spectrometry was used to separate and identify the peptides. The results showed that the use of SPE was an effective method to separate peptides from anthocyanins and other background compounds including high polyphenol content in the juice samples. More than 1000 peptides present in elderberry juice were successfully identified.

Keywords: Anthocyanin sambucus; Elderberry; Orbitrap-MS/MS; Peptidomics; Solid phase extraction.

Publication types

Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Anthocyanins / isolation & purification*
Chromatography, High Pressure Liquid
Peptide Fragments / analysis*
Peptide Fragments / isolation & purification*
Polyphenols / isolation & purification
Sambucus / chemistry*
Solid Phase Extraction / methods*
Tandem Mass Spectrometry

Substances

Anthocyanins
Peptide Fragments
Polyphenols

Grants and funding

P50AT006273/AT/NCCIH NIH HHS/United States