Permanent draft genome sequence of Geobacillus thermocatenulatus strain GS-1

Beiwen Zheng; Fan Zhang; Lujun Chai; Gaoming Yu; Fuchang Shu; Zhengliang Wang; Sanbao Su; Tingsheng Xiang; Zhongzhi Zhang; DuJie Hou; Yuehui She

doi:10.1016/j.margen.2014.09.005

Permanent draft genome sequence of Geobacillus thermocatenulatus strain GS-1

Mar Genomics. 2014 Dec:18PB:129-131. doi: 10.1016/j.margen.2014.09.005. Epub 2014 Oct 1.

Authors

Beiwen Zheng¹, Fan Zhang², Lujun Chai², Gaoming Yu³, Fuchang Shu⁴, Zhengliang Wang⁴, Sanbao Su⁴, Tingsheng Xiang⁴, Zhongzhi Zhang⁵, DuJie Hou², Yuehui She⁶

Affiliations

¹ State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Zhejiang University, Hangzhou, China.
² The Key Laboratory of Marine Reservoir Evolution and Hydrocarbon Accumulation Mechanism, School of Energy Resources, China University of Geosciences, Beijing, China.
³ College of Petroleum Engineering, Yangtze University, Jingzhou, China.
⁴ College of Chemistry and Environmental Engineering, Yangtze University, Jingzhou, China.
⁵ State Key Laboratory of Heavy Oil Processing, China University of Petroleum, Beijing, China.
⁶ College of Chemistry and Environmental Engineering, Yangtze University, Jingzhou, China. Electronic address: sheyuehui@263.net.

PMID: 25280889
DOI: 10.1016/j.margen.2014.09.005

Abstract

Geobacillus thermocatenulatus strain GS-1 is a thermophilic bacillus having a growth optimum at 60°C, capable of degrading alkanes. It was isolated from the formation water of a high-temperature deep oil reservoir in Qinghai oilfield, China. Here, we report the draft genome sequence with an estimated assembly size of 3.5Mb. A total of 3371 protein-coding sequences, including monooxygenase, alcohol dehydrogenase, aldehyde dehydrogenase, fatty acid-CoA ligase, acyl-CoA dehydrogenase, enoyl-CoA hydrogenase, hydroxyacyl-CoA dehydrogenase and thiolase, were detected in the genome, which are involved in the alkane degradation pathway. Our results may provide insights into the genetic basis of the adaptation of this strain to high-temperature oilfield ecosystems.

Keywords: Genome; Geobacillus thermocatenulatus; Hydrocarbon degradation; Thermophile.