Differential expression of VEGF and IL-1alpha after photodynamic treatment in combination with doxorubicin or taxotere

Anticancer Res. 2014 Oct;34(10):5295-302.

Abstract

Aim: To show the impact of chemotherapeutic drugs doxorubicin and taxotere on the molecular pattern of cell response to photodynamic treatment (PDT).

Materials and methods: Human squamous cell carcinoma cells A-431 were studied. Apoptosis was investigated by recording caspase-3 activity. Expression of IL-1alpha and VEGF on mRNA and protein levels was measured by qPCR and ELISA.

Results: PDT in combination with either doxorubicin or taxotere was found to be more cytotoxic in comparison to either single-treatment. The expression of IL-1alpha and VEGF was up-regulated in PDT-treated cells, either alone or in combination with doxorubicin or taxotere. Addition of doxorubicin to the cytokine induction after PDT was not detected, however, taxotere promoted significant over-expression of IL-1alpha and VEGF on the protein level.

Conclusion: Contribution of chemotherapeutic drugs to IL-1 alpha and VEGF release from cells which received dual treatment involving PDT could be significantly different, despite the same level of cytotoxicity.

Keywords: IL-1alpha; Photodynamic therapy; VEGF; doxorubicin; mTHPC; taxotere.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibiotics, Antineoplastic / pharmacology
  • Antibiotics, Antineoplastic / toxicity
  • Cell Line, Tumor
  • Cell Survival / drug effects
  • Cell Survival / genetics
  • Docetaxel
  • Dose-Response Relationship, Drug
  • Doxorubicin / pharmacology*
  • Doxorubicin / toxicity
  • Gene Expression Regulation, Neoplastic / drug effects*
  • Humans
  • Interleukin-1alpha / genetics*
  • Photochemotherapy
  • Taxoids / pharmacology*
  • Taxoids / toxicity
  • Vascular Endothelial Growth Factor A / genetics*

Substances

  • Antibiotics, Antineoplastic
  • Interleukin-1alpha
  • Taxoids
  • Vascular Endothelial Growth Factor A
  • Docetaxel
  • Doxorubicin