[Expression of the Bacillus pumilus chloramphenicol acetyltransferase gene in Bacillus subtilis, achieved by the P-R-promotor of phage lambda]

E V Luk'ianov; M L Chikindas; A I Stepanov

[Expression of the Bacillus pumilus chloramphenicol acetyltransferase gene in Bacillus subtilis, achieved by the P-R-promotor of phage lambda]

Genetika. 1989 Mar;25(3):555-6.

[Article in Russian]

Authors

E V Luk'ianov, M L Chikindas, A I Stepanov

PMID: 2527182

Abstract

The possibility of expression of the Bacillus pumilus chloramphenicol acetyltransferase gene (cat) in Bacillus subtilis from the pR promoter of phage lambda has been investigated in this work. For this purpose, the plasmid pPL703 carrying the B. pumilus DNA segment with the cat gene lacking promoter has been combined with the plasmid pBM21 containing the pR promoter. The recombinant plasmid pEL1 is capable of providing the 60 mkg/ml chloramphenicol resistance in Bac. subtilis cells.

MeSH terms

Bacillus / genetics*
Bacillus subtilis / genetics*
Bacteriophage lambda / genetics*
Chloramphenicol O-Acetyltransferase / genetics*
Gene Expression Regulation*
Genes, Bacterial
Plasmids
Promoter Regions, Genetic

Substances

Chloramphenicol O-Acetyltransferase