Evaluation of fibroblasts adhesion and proliferation on alginate-gelatin crosslinked hydrogel

Bapi Sarker; Raminder Singh; Raquel Silva; Judith A Roether; Joachim Kaschta; Rainer Detsch; Dirk W Schubert; Iwona Cicha; Aldo R Boccaccini

doi:10.1371/journal.pone.0107952

Evaluation of fibroblasts adhesion and proliferation on alginate-gelatin crosslinked hydrogel

PLoS One. 2014 Sep 30;9(9):e107952. doi: 10.1371/journal.pone.0107952. eCollection 2014.

Authors

Bapi Sarker¹, Raminder Singh², Raquel Silva¹, Judith A Roether³, Joachim Kaschta³, Rainer Detsch¹, Dirk W Schubert³, Iwona Cicha⁴, Aldo R Boccaccini¹

Affiliations

¹ Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, Erlangen, Germany.
² Department of Cardiology and Angiology, University Hospital Erlangen, Erlangen, Germany.
³ Institute of Polymer Materials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, Erlangen, Germany.
⁴ Department of Cardiology and Angiology, University Hospital Erlangen, Erlangen, Germany; Cardiovascular Nanomedicine Unit, Section of Experimental Oncology and Nanomedicine, ENT Department, University Hospital, Erlangen, Germany.

Abstract

Due to the relatively poor cell-material interaction of alginate hydrogel, alginate-gelatin crosslinked (ADA-GEL) hydrogel was synthesized through covalent crosslinking of alginate di-aldehyde (ADA) with gelatin that supported cell attachment, spreading and proliferation. This study highlights the evaluation of the physico-chemical properties of synthesized ADA-GEL hydrogels of different compositions compared to alginate in the form of films. Moreover, in vitro cell-material interaction on ADA-GEL hydrogels of different compositions compared to alginate was investigated by using normal human dermal fibroblasts. Viability, attachment, spreading and proliferation of fibroblasts were significantly increased on ADA-GEL hydrogels compared to alginate. Moreover, in vitro cytocompatibility of ADA-GEL hydrogels was found to be increased with increasing gelatin content. These findings indicate that ADA-GEL hydrogel is a promising material for the biomedical applications in tissue-engineering and regeneration.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alginates / chemistry
Alginates / pharmacology*
Biocompatible Materials
Cell Adhesion / drug effects
Cell Proliferation / drug effects
Cell Survival / drug effects
Fibroblasts / drug effects*
Fibroblasts / metabolism
Gelatin / chemistry
Gelatin / pharmacology*
Humans
Hydrogels / chemistry
Hydrogels / pharmacology*
Mitochondria / drug effects
Mitochondria / metabolism
Primary Cell Culture
Tissue Engineering
Tissue Scaffolds*

Substances

Alginates
Biocompatible Materials
Hydrogels
Gelatin

Grants and funding

This work was supported by the Emerging Fields Initiative (EFI) of the University of Erlangen-Nuremberg (project TOPbiomat) (http://www.efi.uni-erlangen.org/projects/topbiomat/) and the German Academic Exchange Service (DAAD) (https://www.daad.de/en/). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.