Abstract
Six isolates recovered from coffee seeds giving off a potato-like flavour were studied. Gene sequencing (rrs and rpoB) showed they belong to the genus Pantoea. By DNA-DNA hybridization, the isolates constituted a genomic species with less than 17% relatedness to 96 strains representing enterobacterial species. Multilocus sequence analysis (gyrB, rpoB, atpD and infB genes) showed the isolates to represent a discrete species of the genus Pantoea. Nutritional versatility of the novel species was poor. The novel species is proposed as Pantoea coffeiphila sp.nov. and its type strain is Ca04(T) ( =CIP 110718(T) =DSM 28482(T)).
© 2015 IUMS.
MeSH terms
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Bacterial Typing Techniques
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Burundi
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Coffee / microbiology*
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DNA, Bacterial / genetics
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Food Contamination
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Food Microbiology*
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Genes, Bacterial
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Molecular Sequence Data
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Multilocus Sequence Typing
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Nucleic Acid Hybridization
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Pantoea / classification*
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Pantoea / genetics
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Pantoea / isolation & purification
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Phylogeny*
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RNA, Ribosomal, 16S / genetics
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Sequence Analysis, DNA
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Taste*
Substances
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Coffee
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DNA, Bacterial
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RNA, Ribosomal, 16S
Associated data
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GENBANK/KJ427825
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GENBANK/KJ427826
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GENBANK/KJ427827
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GENBANK/KJ427828
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GENBANK/KJ427829
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GENBANK/KJ427830
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GENBANK/KJ427831
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GENBANK/KJ427832
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GENBANK/KM205360
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GENBANK/KM205361
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GENBANK/KM205362
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GENBANK/KM205363
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GENBANK/KM205364
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GENBANK/KM205365
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GENBANK/KM205366
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GENBANK/KM205367
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GENBANK/KM205368
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GENBANK/KM205369
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GENBANK/KM205370
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GENBANK/KM205371