Pantoea coffeiphila sp. nov., cause of the 'potato taste' of Arabica coffee from the African Great Lakes region

Int J Syst Evol Microbiol. 2015 Jan;65(Pt 1):23-29. doi: 10.1099/ijs.0.063545-0. Epub 2014 Sep 29.

Abstract

Six isolates recovered from coffee seeds giving off a potato-like flavour were studied. Gene sequencing (rrs and rpoB) showed they belong to the genus Pantoea. By DNA-DNA hybridization, the isolates constituted a genomic species with less than 17% relatedness to 96 strains representing enterobacterial species. Multilocus sequence analysis (gyrB, rpoB, atpD and infB genes) showed the isolates to represent a discrete species of the genus Pantoea. Nutritional versatility of the novel species was poor. The novel species is proposed as Pantoea coffeiphila sp.nov. and its type strain is Ca04(T) ( =CIP 110718(T) =DSM 28482(T)).

MeSH terms

  • Bacterial Typing Techniques
  • Burundi
  • Coffee / microbiology*
  • DNA, Bacterial / genetics
  • Food Contamination
  • Food Microbiology*
  • Genes, Bacterial
  • Molecular Sequence Data
  • Multilocus Sequence Typing
  • Nucleic Acid Hybridization
  • Pantoea / classification*
  • Pantoea / genetics
  • Pantoea / isolation & purification
  • Phylogeny*
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Taste*

Substances

  • Coffee
  • DNA, Bacterial
  • RNA, Ribosomal, 16S

Associated data

  • GENBANK/KJ427825
  • GENBANK/KJ427826
  • GENBANK/KJ427827
  • GENBANK/KJ427828
  • GENBANK/KJ427829
  • GENBANK/KJ427830
  • GENBANK/KJ427831
  • GENBANK/KJ427832
  • GENBANK/KM205360
  • GENBANK/KM205361
  • GENBANK/KM205362
  • GENBANK/KM205363
  • GENBANK/KM205364
  • GENBANK/KM205365
  • GENBANK/KM205366
  • GENBANK/KM205367
  • GENBANK/KM205368
  • GENBANK/KM205369
  • GENBANK/KM205370
  • GENBANK/KM205371