Complete genome sequence of three tomato ringspot virus isolates: evidence for reassortment and recombination

Arch Virol. 2015 Feb;160(2):543-7. doi: 10.1007/s00705-014-2240-y. Epub 2014 Oct 1.

Abstract

The genome sequence of tomato ringspot virus (ToRSV, a subgroup C nepovirus) is currently available for one raspberry isolate. In this study, we describe the complete genome sequence of three additional isolates from raspberry (Rasp1-2014), grapevine (GYV-2014) and prunus (13C280). The degree of nucleotide sequence identity shared between RNA1 and RNA2 in the 5'-terminal 900 nucleotides and 3' untranslated region varied from 98-99 % (13C280, GYV-2014) to 80 % (Rasp1-2014). Phylogenetic studies revealed distinct origins for Rasp1-2014 RNA1 and RNA2, suggesting reassortment. Two recombination events were also identified in the 3' UTR and 5'-terminal region of RNA1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3' Untranslated Regions / genetics
  • 5' Untranslated Regions / genetics
  • Amino Acid Sequence
  • Base Sequence
  • Genome, Viral / genetics*
  • Molecular Sequence Data
  • Nepovirus / classification
  • Nepovirus / genetics*
  • Nepovirus / isolation & purification
  • Phylogeny
  • Plant Diseases / virology
  • Prunus / virology*
  • RNA, Viral / genetics
  • Reassortant Viruses / classification
  • Reassortant Viruses / genetics*
  • Reassortant Viruses / isolation & purification
  • Recombination, Genetic*
  • Rubus / virology*
  • Sequence Alignment
  • Sequence Analysis, RNA
  • Sequence Homology, Amino Acid
  • Viral Proteins / genetics
  • Vitis / virology*

Substances

  • 3' Untranslated Regions
  • 5' Untranslated Regions
  • RNA, Viral
  • Viral Proteins

Associated data

  • GENBANK/KM083890
  • GENBANK/KM083891
  • GENBANK/KM083892
  • GENBANK/KM083893
  • GENBANK/KM083894
  • GENBANK/KM083895